Nucleosomes block access to DNA methyltransferase, unless they are remodeled by DECREASE in DNA METHYLATION 1 (DDM1/Lsh/HELLS), a Snf2-like master regulator of epigenetic inheritance. We show that DDM1 promotes replacement of histone variant H3.3 by …
Quick Reference for cryo-EM data acquisition using EPU on the Titan Krios
The Piwi-interacting RNA (piRNA) pathway safeguards genomic integrity by silencing transposable elements (transposons) in the germline. While Piwi is the central piRNA factor, others including Asterix/Gtsf1 have also been demonstrated to be critical …
Quick Reference for cryo-EM data acquisition using EPU on the Titan Krios
Step-by-step notes for cryo-EM sample preparation and loading
Quick Reference for Changing Energy on the Titan Krios